ABOU HAMMOUD, Aya; GIRAUD, Julie; GAUTHEREAU, Xavier; BLANCHARD, Camille; DABURON, Sophie; ZESE, Marco; MOLINA-CASTRO, Silvia; DUBUS, Pierre; VARON, Christine; BOEUF, Helene

doi:10.3390/biomedicines11123293

Bioingénierie tissulaire [BIOTIS]
BoRdeaux Institute in onCology [Inserm U1312 - BRIC]

GIRAUD, Julie

Immunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
BoRdeaux Institute in onCology [Inserm U1312 - BRIC]

GAUTHEREAU, Xavier
Immunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]

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Article de revue

Ce document a été publié dans

Biomedicines. 2023, vol. 11, n° 12

Résumé en anglais

Cancer stem cells are a subpopulation of tumor cells characterized by their ability to self-renew, induce tumors upon engraftment in animals and exhibit strong resistance to chemotherapy and radiotherapy. These cells exhibit numerous characteristics in common with embryonic stem cells, expressing some of their markers, typically absent in non-pathological adult differentiated cells. The aim of this study was to investigate the potential of conditioned media from cancer stem cells to modulate the fate of Leukemia Inhibitory Factor (LIF)-dependent murine embryonic stem cells (mESCs) as a way to obtain a direct readout of the secretome of cancer cells. A functional assay, “the StemDif sensor test”, was developed with two types of cancer stem cells derived from grade IV glioblastoma (adult and pediatric) or from gastric adenocarcinoma. We show that conditioned media from the selection of adult but not pediatric Glioma-Inducing Cells (GICs) maintain mESCs’ pluripotency in correlation with LIF secretion and activation of STAT3 protein. In contrast, conditioned media from gastric adenocarcinoma cells display LIF-independent stemness and differentiation activities on mESC. Our test stands out for its user-friendly procedures, affordability and straightforward output, positioning it as a pioneering tool for in-depth exploration of cancer stem cell secretome characteristics. © 2023 by the authors.< Réduire

Mots clés en anglais

secretome

conditioned media

paracrine

tumor

cancer stem cells

LIF

mESCs

URI

https://oskar-bordeaux.fr/handle/20.500.12278/199713

DOI

http://dx.doi.org/10.3390/biomedicines11123293

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The “StemDif Sensor Test”: A Straightforward, Non-Invasive Assay to Characterize the Secreted Stemness and/or Differentiation Activities of Tumor-Derived Cancer Cell Lines

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The “StemDif Sensor Test”: A Straightforward, Non-Invasive Assay to Characterize the Secreted Stemness and/or Differentiation Activities of Tumor-Derived Cancer Cell Lines

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Ce document a été publié dans

Résumé en anglais

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URI

DOI

Unités de recherche