DAO, Tuyen P. T.; FERNANDES, Fabio; IBARBOURE, Emmanuel; FERJI, Khalid; PRIETO, Manuel; SANDRE, Olivier; LE MEINS, Jean-Francois

doi:10.1039/C6SM01625A

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DAO, Tuyen P. T.
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

FERNANDES, Fabio
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol

IBARBOURE, Emmanuel
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

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Article de revue

Ce document a été publié dans

Soft Matter. 2017, vol. 13, n° 3, p. 627-637

Royal Society of Chemistry

Résumé en anglais

Phase separation in hybrid polymer/lipid giant unillamelar vesicles (GHUVs) has been described over the last few years. However there is still a lack of understanding in physical and molecular factors governing the phase separation in such systems. Among these parameters it has been suggested that in analogy to multicomponent lipid vesicles that hydrophobic mismatchs as well as lipid fludity play a role.In this work, we aim to map a global picture of phase separation and domain formation in membrane of GHUVs by using various copolymers based on poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) (PEO) with different architectures (grafted, triblock) and molar masses, combined with lipid at fluid (POPC) or gel state (DPPC) at room temperature. From confocal imaging and fluorescence lifetime imaging microscopy technique (FLIM), the phase separation into either micro- or nano- domains within GHVs were studied. In particular, our systematic studies demonstrate that in addition to lipid/polymer fraction or lipid physical state, important factors such as line tension at lipid polymer/lipid boundaries can be finely modulated by the molar mass and architecture of the copolymer and lead to the formation of stable lipid domains with different sizes and morphologies in such GHUVs.< Réduire

Mots clés en anglais

Cryo Electron Microscopy

Hybrid lipid polymer vesicles

Nanodomains

Neutron scattering including small-angle scattering

Membranes bilayers and vesicles

Fluorescence resonance energy transfer FRET

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19959

DOI

http://dx.doi.org/10.1039/C6SM01625A

Project ANR

Cinétique de Translocation de Particules à travers de Bicouches Auto-Assemblées - ANR-12-BS08-0018

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629