Modulation of phase separation at micron scale and nanoscale in hybrid polymer/lipid giant unilamellar vesicles (GHUVs)
DAO, Tuyen P. T.
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
FERNANDES, Fabio
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
IBARBOURE, Emmanuel
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
See more >
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
DAO, Tuyen P. T.
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
FERNANDES, Fabio
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
IBARBOURE, Emmanuel
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
FERJI, Khalid
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
PRIETO, Manuel
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
SANDRE, Olivier
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
LE MEINS, Jean-Francois
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
< Reduce
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Language
en
Article de revue
This item was published in
Soft Matter. 2017, vol. 13, n° 3, p. 627-637
Royal Society of Chemistry
English Abstract
Phase separation in hybrid polymer/lipid giant unillamelar vesicles (GHUVs) has been described over the last few years. However there is still a lack of understanding in physical and molecular factors governing the phase ...Read more >
Phase separation in hybrid polymer/lipid giant unillamelar vesicles (GHUVs) has been described over the last few years. However there is still a lack of understanding in physical and molecular factors governing the phase separation in such systems. Among these parameters it has been suggested that in analogy to multicomponent lipid vesicles that hydrophobic mismatchs as well as lipid fludity play a role.In this work, we aim to map a global picture of phase separation and domain formation in membrane of GHUVs by using various copolymers based on poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) (PEO) with different architectures (grafted, triblock) and molar masses, combined with lipid at fluid (POPC) or gel state (DPPC) at room temperature. From confocal imaging and fluorescence lifetime imaging microscopy technique (FLIM), the phase separation into either micro- or nano- domains within GHVs were studied. In particular, our systematic studies demonstrate that in addition to lipid/polymer fraction or lipid physical state, important factors such as line tension at lipid polymer/lipid boundaries can be finely modulated by the molar mass and architecture of the copolymer and lead to the formation of stable lipid domains with different sizes and morphologies in such GHUVs.Read less <
English Keywords
Cryo Electron Microscopy
Hybrid lipid polymer vesicles
Nanodomains
Neutron scattering including small-angle scattering
Membranes bilayers and vesicles
Fluorescence resonance energy transfer FRET
ANR Project
Cinétique de Translocation de Particules à travers de Bicouches Auto-Assemblées - ANR-12-BS08-0018
Origin
Hal imported