NGUYEN, Vo-Thu; DE PAUW-GILLET, Marie-Claire; GAUTHIER, Mario; SANDRE, Olivier

doi:10.3390/nano8121014

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NGUYEN, Vo-Thu
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
University of Waterloo [Waterloo]

DE PAUW-GILLET, Marie-Claire
Laboratory of Mammalian Cell Culture [GIGA-R]
Université de Liège

GAUTHIER, Mario
University of Waterloo [Waterloo]

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Article de revue

Ce document a été publié dans

Nanomaterials. 2018, vol. 8, n° 12, p. 1014

MDPI

Résumé en anglais

Magnetic nanoparticles (MNPs) of magnetite (Fe3O4) were prepared using a polystyrene-graft-poly(2-vinylpyridine) copolymer (denoted G0PS-g-P2VP or G1) as template. These MNPs were subjected to self-assembly with a poly(acrylic acid)-block-poly(2-hydroxyethyl acrylate) double-hydrophilic block copolymer (DHBC), PAA-b-PHEA, to form water-dispersible magnetic polyion complex (MPIC) micelles. Large Fe3O4 crystallites were visualized by transmission electron microscopy (TEM) and magnetic suspensions of MPIC micelles exhibited improved colloidal stability in aqueous environments over a wide pH and ionic strength range. Biological cells incubated for 48 h with MPIC micelles at the highest concentration (1250 µg of Fe3O4 per mL) had a cell viability of 91%, as compared with 51% when incubated with bare (unprotected) MNPs. Cell internalization, visualized by confocal laser scanning microscopy (CLSM) and TEM, exhibited strong dependence on the MPIC micelle concentration and incubation time, as also evidenced by fluorescence-activated cell sorting (FACS). The usefulness of MPIC micelles for cellular radiofrequency magnetic field hyperthermia (MFH) was also confirmed, as the MPIC micelles showed a dual dose-dependent effect (concentration and duration of magnetic field exposure) on the viability of L929 mouse fibroblasts and U87 human glioblastoma epithelial cells.< Réduire

Mots clés en anglais

magnetic field hyperthermia

cell internalization

cytotoxicity

double-hydrophilic block copolymer

arborescent polymer

Magnetic Intra-Lysosomal Hyperthermia

magnetic nanoparticles

magnetic polyion complex micelle

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19940

DOI

http://dx.doi.org/10.3390/nano8121014

Projet Européen

Erasmus Mundus - International Doctoral School in Functional Materials
Multifunctional Nanoparticles for Magnetic Hyperthermia and Indirect Radiation Therapy

Project ANR

Magnéto-Chimiothérapie : Modélisation de la Délivrance Induite par Champ Magnétique Radiofréquence d'Anticancéreux par des Nano-Vésicules Polymères et Suivi par IRM d'un Modèle de Glioblastome - ANR-13-BS08-0017

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629