SPONSEL, Janina; GUO, Yubing; HAMZAM, Lutfir; LAVANANT, Alice; PÉREZ-RIVERÓN, Annia; PARTIOT, Emma; MULLER, Quentin; ROTTURA, Julien; GAUDIN, Raphael; HAUCK, Dirk; TITZ, Alexander; FLACHER, Vincent; RÖMER, Winfried; MUELLER, Christopher

doi:10.15252/embr.202255971

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SPONSEL, Janina
Institut de biologie moléculaire et cellulaire [IBMC]
Albert-Ludwigs-Universität Freiburg = University of Freiburg

GUO, Yubing
Institut de biologie moléculaire et cellulaire [IBMC]

HAMZAM, Lutfir
Institut de biologie moléculaire et cellulaire [IBMC]

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Article de revue

Ce document a été publié dans

EMBO Reports. 2023, vol. 24, n° 4, p. e55971

Résumé en anglais

Abstract Pseudomonas aeruginosa is a Gram‐negative bacterium causing morbidity and mortality in immuno‐compromised humans. It produces a lectin, LecB, that is considered a major virulence factor, however, its impact on the immune system remains incompletely understood. Here we show that LecB binds to endothelial cells in human skin and mice and disrupts the transendothelial passage of leukocytes in vitro . It impairs the migration of dendritic cells into the paracortex of lymph nodes leading to a reduced antigen‐specific T cell response. Under the effect of the lectin, endothelial cells undergo profound cellular changes resulting in endocytosis and degradation of the junctional protein VE‐cadherin, formation of an actin rim, and arrested cell motility. This likely negatively impacts the capacity of endothelial cells to respond to extracellular stimuli and to generate the intercellular gaps for allowing leukocyte diapedesis. A LecB inhibitor can restore dendritic cell migration and T cell activation, underlining the importance of LecB antagonism to reactivate the immune response against P. aeruginosa infection.< Réduire

Mots clés en anglais

bacterial lectin

dendritic cells

lymphatics

migration

skin

URI

https://oskar-bordeaux.fr/handle/20.500.12278/199246

DOI

http://dx.doi.org/10.15252/embr.202255971

Project ANR

Caractérisation des déterminants moléculaires impliqués dans la migration cellulaire induite par le virus Zika - ANR-20-CE15-0019

Unités de recherche

Bioingénierie Tissulaire (BioTis) - U1026