DRAPPIER, Charlotte; DE OLIVEIRA, Hugo; SANDRE, Olivier; IBARBOURE, Emmanuel; COMBET, Sophie; GARANGER, Elisabeth; LECOMMANDOUX, Sebastien

doi:10.1039/c3fd00098b

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

DRAPPIER, Charlotte
Laboratoire de Chimie des Polymères Organiques [LCPO]

DE OLIVEIRA, Hugo
Laboratoire de Chimie des Polymères Organiques [LCPO]

SANDRE, Olivier
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

Langue

Article de revue

Ce document a été publié dans

Faraday Discussions. 2013, vol. 166, p. 83-100

Royal Society of Chemistry

Résumé en anglais

The aim of this contribution is to design, produce and characterize size-tuneable core-shell micelles from amphiphilic Tat-b-poly(trimethylene carbonate) (Tat-b-PTMC) molecular chimeras, and to explore their biological properties. Because the extensive characterization of nanomaterials is a pre-requisite to understand and rationalize their ensuing properties, we present a detailed description of Tat-b-PTMC micelles thanks to light scattering, AFM imaging and small angle neutron scattering analyses. In vitro, Tat-b-PTMC micelles were found to be rapidly and efficiently internalized by HeLa cells, with cellular uptake kinetics being mostly related to Tat peptide content and, to a lesser extent, to nanoparticle size. We also demonstrated that, after a first membrane-binding step, Tat-b-PTMC micelles were taken up by cells via an energy-dependent endocytotic process.< Réduire

Mots clés en anglais

NANOPARTICLES

DESIGN

DELIVERY

MECHANISM

BLOCK-COPOLYMERS

CELLULAR UPTAKE

TAT PEPTIDE

HIV-1 TAT

SCATTERING

CYTOTOXICITY

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Self-assembled core-shell micelles from peptide-b-polymer molecular chimeras towards structure-activity relationships

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Origine

Unités de recherche