CHERN, Margaret; GRAZON, Chloé; DENNIS, Allison

doi:10.1007/978-1-0716-0463-2_7

Metadatos

Licencia de uso del documento

CHERN, Margaret
Boston University [Boston] [BU]
Division of Materials Science and Engineering

GRAZON, Chloé
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Department of Chemistry, Boston University

DENNIS, Allison
Boston University [Boston] [BU]
Division of Materials Science and Engineering
Department of Biomedical Engineering [Boston]

Idioma

Chapitre d'ouvrage

Este ítem está publicado en

Quantum Dots. 2020, vol. 2135, p. 125-139

Humana, New York, NY

Resumen en inglés

Small, stable, and bright quantum dots (QDs) are of interest in many biosensing and biomedical imaging applications, but current methodologies for obtaining these characteristics can be highly specialized or expensive. We describe a straightforward, low-cost protocol for functionalizing poly(isobutylene-alt-maleic anhydride) (PIMA) with moieties that anchor to the QD surface (histamine), impart hydrophilicity [(2-aminoethyl)trimethylammonium chloride (Me3N+-NH2)], and provide a platform for biofunctionalization via click chemistry (dibenzocyclooctyne (DBCO)). Guidelines to successfully use this polymer for QD ligand exchange are presented, and an example of biofunctionalization with DNA is shown. Stable QD–DNA conjugates are obtained with high yield and without requiring additional purification steps.< Leer menos

Palabras clave en inglés

DNA labeling

Copper-free click chemistry

Biofunctionalization

Nanoparticle

Carboxybetaine

Poly(isobutylene-alt-maleic anhydride)—PIMA

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19700

DOI

http://dx.doi.org/10.1007/978-1-0716-0463-2_7

Proyecto europeo

A Wearable Sensor for Hormones Based on a Native Microbial Sensing

Orígen

Importado de Hal

Centros de investigación

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629