Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy-to-Prepare, Low-Cost Zwitterionic Polymer
GRAZON, Chloé
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Department of Chemistry, Boston University
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Department of Chemistry, Boston University
DENNIS, Allison
Boston University [Boston] [BU]
Division of Materials Science and Engineering
Department of Biomedical Engineering [Boston]
Boston University [Boston] [BU]
Division of Materials Science and Engineering
Department of Biomedical Engineering [Boston]
GRAZON, Chloé
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Department of Chemistry, Boston University
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Department of Chemistry, Boston University
DENNIS, Allison
Boston University [Boston] [BU]
Division of Materials Science and Engineering
Department of Biomedical Engineering [Boston]
< Reduce
Boston University [Boston] [BU]
Division of Materials Science and Engineering
Department of Biomedical Engineering [Boston]
Language
en
Chapitre d'ouvrage
This item was published in
Quantum Dots. 2020, vol. 2135, p. 125-139
Humana, New York, NY
English Abstract
Small, stable, and bright quantum dots (QDs) are of interest in many biosensing and biomedical imaging applications, but current methodologies for obtaining these characteristics can be highly specialized or expensive. We ...Read more >
Small, stable, and bright quantum dots (QDs) are of interest in many biosensing and biomedical imaging applications, but current methodologies for obtaining these characteristics can be highly specialized or expensive. We describe a straightforward, low-cost protocol for functionalizing poly(isobutylene-alt-maleic anhydride) (PIMA) with moieties that anchor to the QD surface (histamine), impart hydrophilicity [(2-aminoethyl)trimethylammonium chloride (Me3N+-NH2)], and provide a platform for biofunctionalization via click chemistry (dibenzocyclooctyne (DBCO)). Guidelines to successfully use this polymer for QD ligand exchange are presented, and an example of biofunctionalization with DNA is shown. Stable QD–DNA conjugates are obtained with high yield and without requiring additional purification steps.Read less <
English Keywords
DNA labeling
Copper-free click chemistry
Biofunctionalization
Nanoparticle
Carboxybetaine
Poly(isobutylene-alt-maleic anhydride)—PIMA
European Project
A Wearable Sensor for Hormones Based on a Native Microbial Sensing
Origin
Hal imported