CHEN, Mingfu; NGUYEN, Thuy; VARONGCHAYAKUL, Nitinun; GRAZON, Chloé; CHERN, Margaret; BAER, R.; LECOMMANDOUX, Sebastien; KLAPPERICH, Catherine; GALAGAN, James; DENNIS, Allison; GRINSTAFF, Mark

doi:10.1002/adhm.202000403

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CHEN, Mingfu
Boston University [Boston] [BU]

NGUYEN, Thuy
Boston University [Boston] [BU]

VARONGCHAYAKUL, Nitinun
Boston University [Boston] [BU]

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Article de revue

Ce document a été publié dans

Advanced Healthcare Materials. 2020, vol. 9, n° 17, p. 2000403

Wiley

Résumé en anglais

Immobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm . The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.< Réduire

Mots clés en anglais

biosensing

Förster resonance energy transfer

quantum dots

transcription factors

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19569

DOI

http://dx.doi.org/10.1002/adhm.202000403

Projet Européen

A Wearable Sensor for Hormones Based on a Native Microbial Sensing

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629