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Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing

dc.rights.licenseopen
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorCHEN, Mingfu
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorNGUYEN, Thuy
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorVARONGCHAYAKUL, Nitinun
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGRAZON, Chloé
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorCHERN, Margaret
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorBAER, R.
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorKLAPPERICH, Catherine
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorGALAGAN, James
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorDENNIS, Allison
hal.structure.identifierBoston University [Boston] [BU]
dc.contributor.authorGRINSTAFF, Mark
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2020
dc.identifier.issn2192-2640
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19569
dc.description.abstractEnImmobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm . The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.
dc.language.isoen
dc.publisherWiley
dc.subject.enbiosensing
dc.subject.enFörster resonance energy transfer
dc.subject.enquantum dots
dc.subject.entranscription factors
dc.title.enSurface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
dc.typeArticle de revue
dc.identifier.doi10.1002/adhm.202000403
dc.subject.halChimie/Polymères
dc.subject.halChimie/Matériaux
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halSciences de l'ingénieur [physics]/Optique / photonique
dc.description.sponsorshipEuropeA Wearable Sensor for Hormones Based on a Native Microbial Sensing
bordeaux.journalAdvanced Healthcare Materials
bordeaux.page2000403
bordeaux.volume9
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue17
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02904157
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02904157v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Advanced%20Healthcare%20Materials&rft.date=2020&rft.volume=9&rft.issue=17&rft.spage=2000403&rft.epage=2000403&rft.eissn=2192-2640&rft.issn=2192-2640&rft.au=CHEN,%20Mingfu&NGUYEN,%20Thuy&VARONGCHAYAKUL,%20Nitinun&GRAZON,%20Chlo%C3%A9&CHERN,%20Margaret&rft.genre=article


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