CHEN, Mingfu; NGUYEN, Thuy T.; VARONGCHAYAKUL, Nitinun; GRAZON, Chloé; CHERN, Margaret; BAER, R. C.; LECOMMANDOUX, Sebastien; KLAPPERICH, Catherine M.; GALAGAN, James E.; DENNIS, Allison M.; GRINSTAFF, Mark W.

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CHEN, Mingfu
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]

NGUYEN, Thuy T.
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]

VARONGCHAYAKUL, Nitinun
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]

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Article de revue

Ce document a été publié dans

Advanced Healthcare Materials. 2020, vol. 9, n° 17, p. 2000403

Wiley

Date de soutenance

2020

Résumé en anglais

Immobilization of biosensors on surfaces is a key step towards development of devices for real world applications. Here we describe the preparation, characterization, and evaluation of a surface bound transcription factor – nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot. Upon addition of anhydrotetracycline (aTc) – the analyte – the TetR-QDs release from the surface-bound DNA, resulting in loss of the Förster resonance energy transfer (FRET) signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µM aTc with a limit of detection of 80 nM. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.< Réduire

Mots clés en anglais

biosensing

transcription factor

quantum dots

Förster resonance energy transfer

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19551

Projet Européen

A Wearable Sensor for Hormones Based on a Native Microbial Sensing

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629