Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
VARONGCHAYAKUL, Nitinun
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]
See more >
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]
VARONGCHAYAKUL, Nitinun
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]
GRAZON, Chloé
Boston University [Boston] [BU]
Department of Chemistry and Chemical Biology [Boston]
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Boston University [Boston] [BU]
Department of Chemistry and Chemical Biology [Boston]
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
BAER, R. C.
Boston University [Boston] [BU]
National Emerging Infectious Diseases Laboratories [NEIDL]
Boston University [Boston] [BU]
National Emerging Infectious Diseases Laboratories [NEIDL]
LECOMMANDOUX, Sebastien
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
KLAPPERICH, Catherine M.
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]
Boston University [Boston] [BU]
Department of Biomedical Engineering [Boston]
GRINSTAFF, Mark W.
Boston University [Boston] [BU]
Department of Chemistry and Chemical Biology [Boston]
< Reduce
Boston University [Boston] [BU]
Department of Chemistry and Chemical Biology [Boston]
Language
en
Article de revue
This item was published in
Advanced Healthcare Materials. 2020, vol. 9, n° 17, p. 2000403
Wiley
Date
2020English Abstract
Immobilization of biosensors on surfaces is a key step towards development of devices for real world applications. Here we describe the preparation, characterization, and evaluation of a surface bound transcription factor ...Read more >
Immobilization of biosensors on surfaces is a key step towards development of devices for real world applications. Here we describe the preparation, characterization, and evaluation of a surface bound transcription factor – nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot. Upon addition of anhydrotetracycline (aTc) – the analyte – the TetR-QDs release from the surface-bound DNA, resulting in loss of the Förster resonance energy transfer (FRET) signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µM aTc with a limit of detection of 80 nM. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.Read less <
English Keywords
biosensing
transcription factor
quantum dots
Förster resonance energy transfer
European Project
A Wearable Sensor for Hormones Based on a Native Microbial Sensing
Origin
Hal imported