SILVE, Aude; LERAY, Isabelle; LEGUÈBE, Michael; POIGNARD, Clair; MIR, Lluis M.

doi:10.1016/j.bioelechem.2015.06.002

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SILVE, Aude
Institute for Pulsed Power and Microwave Technology [Eggenstein-Leopoldshafen]

LERAY, Isabelle
Vectorologie et thérapeutiques anti-cancéreuses [Villejuif] [UMR 8203]
Université Paris-Sud - Paris 11 [UP11]

LEGUÈBE, Michael
Modélisation Mathématique pour l'Oncologie [MONC]

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Bioelectrochemistry. 2015-12, vol. Volume 106, Part B, December 2015, Pages 369–378

Elsevier

Résumé en anglais

Electric pulses of a few nanoseconds in duration can induce reversible permeabilization of cell membrane and cell death. Whether these effects are caused by ionic or purely dielectric phenomena is still discussed. We address this question by studying the impact of conductivity of the pulsing buffer on the effect of pulses of 12 ns and 3.2 MV/m on the DC-3F mammalian cell line. When pulses were applied in a high-conductivity medium (1.5 S/m), cells experienced both reversible electropermeabilization and cell death. On the contrary, no effect was observed in the low-conductivity medium (0.1 S/m). Possible artifacts due to differences in viscosity, temperature increase or electrochemical reactions were excluded. The influence of conductivity reported here suggests that charges still play a role, even for 12-ns pulses. All theoretical models agree with this experimental observation, since all suggest that only high-conductivity medium can induce a transmembrane voltage high enough to induce pore creation, in turn. However, most models fail to describe why pulse accumulation is experimentally required to observe biological effects. They mostly show no increase of permeabilization with accumulation of pulses. Currently, only one model properly describes pulse accumulation by modeling diffusion of the altered membrane regions.< Réduire

Mots clés en anglais

Nanosecond-pulsed electric fields

Temperature

Medium conductivity

Electroporation

Electropermeabilization

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Cell membrane permeabilization by 12-ns electric pulses: Not a purely dielectric, but a charge-dependent phenomenon

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