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Cell membrane permeabilization by 12-ns electric pulses: Not a purely dielectric, but a charge-dependent phenomenon

hal.structure.identifierInstitute for Pulsed Power and Microwave Technology [Eggenstein-Leopoldshafen]
dc.contributor.authorSILVE, Aude
hal.structure.identifierVectorologie et thérapeutiques anti-cancéreuses [Villejuif] [UMR 8203]
hal.structure.identifierUniversité Paris-Sud - Paris 11 [UP11]
dc.contributor.authorLERAY, Isabelle
hal.structure.identifierModélisation Mathématique pour l'Oncologie [MONC]
dc.contributor.authorLEGUÈBE, Michael
hal.structure.identifierModélisation Mathématique pour l'Oncologie [MONC]
dc.contributor.authorPOIGNARD, Clair
hal.structure.identifierVectorologie et thérapeutiques anti-cancéreuses [Villejuif] [UMR 8203]
hal.structure.identifierUniversité Paris-Sud - Paris 11 [UP11]
dc.contributor.authorMIR, Lluis M.
dc.date.accessioned2024-04-04T03:17:33Z
dc.date.available2024-04-04T03:17:33Z
dc.date.issued2015-12
dc.identifier.issn1567-5394
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/194315
dc.description.abstractEnElectric pulses of a few nanoseconds in duration can induce reversible permeabilization of cell membrane and cell death. Whether these effects are caused by ionic or purely dielectric phenomena is still discussed. We address this question by studying the impact of conductivity of the pulsing buffer on the effect of pulses of 12 ns and 3.2 MV/m on the DC-3F mammalian cell line. When pulses were applied in a high-conductivity medium (1.5 S/m), cells experienced both reversible electropermeabilization and cell death. On the contrary, no effect was observed in the low-conductivity medium (0.1 S/m). Possible artifacts due to differences in viscosity, temperature increase or electrochemical reactions were excluded. The influence of conductivity reported here suggests that charges still play a role, even for 12-ns pulses. All theoretical models agree with this experimental observation, since all suggest that only high-conductivity medium can induce a transmembrane voltage high enough to induce pore creation, in turn. However, most models fail to describe why pulse accumulation is experimentally required to observe biological effects. They mostly show no increase of permeabilization with accumulation of pulses. Currently, only one model properly describes pulse accumulation by modeling diffusion of the altered membrane regions.
dc.language.isoen
dc.publisherElsevier
dc.subject.enNanosecond-pulsed electric fields
dc.subject.enTemperature
dc.subject.enMedium conductivity
dc.subject.enElectroporation
dc.subject.enElectropermeabilization
dc.title.enCell membrane permeabilization by 12-ns electric pulses: Not a purely dielectric, but a charge-dependent phenomenon
dc.typeArticle de revue
dc.identifier.doi10.1016/j.bioelechem.2015.06.002
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biophysique
dc.subject.halMathématiques [math]/Equations aux dérivées partielles [math.AP]
bordeaux.journalBioelectrochemistry
bordeaux.volumeVolume 106, Part B, December 2015, Pages 369–378
bordeaux.hal.laboratoriesInstitut de Mathématiques de Bordeaux (IMB) - UMR 5251*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01203284
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01203284v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Bioelectrochemistry&rft.date=2015-12&rft.volume=Volume%20106,%20Part%20B,%20December%202015,%20Pages%20369%E2%80%93378&rft.eissn=1567-5394&rft.issn=1567-5394&rft.au=SILVE,%20Aude&LERAY,%20Isabelle&LEGU%C3%88BE,%20Michael&POIGNARD,%20Clair&MIR,%20Lluis%20M.&rft.genre=article


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