Real-Time Assessment of Ultrasound-Mediated Drug Delivery Using Fibered Confocal Fluorescence Microscopy
DERIEPPE, Marc
Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
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Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
DERIEPPE, Marc
Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
DENIS DE SENNEVILLE, Baudouin
Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
Institut de Mathématiques de Bordeaux [IMB]
Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
Institut de Mathématiques de Bordeaux [IMB]
MOONEN, Chrit
Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
< Réduire
Imaging Division
Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
Langue
en
Article de revue
Ce document a été publié dans
Molecular Imaging and Biology. 2013-02-01, vol. 15, n° 1, p. 3-11
Springer Verlag
Résumé en anglais
Purpose: Transport across the plasma membrane is a critical step of drug delivery for weakly permeable compounds with intracellular mode of action. The purpose of this study is to demonstrate real-time monitoring of ...Lire la suite >
Purpose: Transport across the plasma membrane is a critical step of drug delivery for weakly permeable compounds with intracellular mode of action. The purpose of this study is to demonstrate real-time monitoring of ultrasound (US)-mediated cell-impermeable model drug uptake with fibered confocal fluorescence microscopy (FCFM). Procedures: An in vitro setup was designed to combine a mono-element US transducer, a cell chamber with a monolayer of tumor cells together with SonoVue microbubbles, and a FCFM system. The cell-impermeable intercalating dye, SYTOX Green, was used to monitor US-mediated uptake. Results: The majority of the cell population showed fluorescence signal enhancement 10 s after US onset. The mean rate constant k of signal enhancement was calculated to be 0.23±0.04 min −1. Conclusions: Feasibility of real-time monitoring of US-mediated intracellular delivery by FCFM has been demonstrated. The method allowed quantitative assessment of model drug uptake, holding great promise for further local drug delivery studies.< Réduire
Mots clés en anglais
Drug delivery
Biological barrier
Plasma membrane permeabilization
Ultrasound
bioeffects
Fibered confocal fluorescence microscopy
Pharmacokinetic parameters
SYTOX
Green
US-mediated drug delivery
Origine
Importé de halUnités de recherche