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Real-Time Assessment of Ultrasound-Mediated Drug Delivery Using Fibered Confocal Fluorescence Microscopy

hal.structure.identifierImaging Division
hal.structure.identifierImagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
dc.contributor.authorDERIEPPE, Marc
hal.structure.identifierImagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
dc.contributor.authorYUDINA, Anna
hal.structure.identifierImagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
dc.contributor.authorLEPETIT-COIFFÉ, Matthieu
hal.structure.identifierImaging Division
hal.structure.identifierImagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorDENIS DE SENNEVILLE, Baudouin
hal.structure.identifierImaging Division
dc.contributor.authorBOS, Clemens
hal.structure.identifierImaging Division
hal.structure.identifierImagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
dc.contributor.authorMOONEN, Chrit
dc.date.accessioned2024-04-04T03:07:03Z
dc.date.available2024-04-04T03:07:03Z
dc.date.issued2013-02-01
dc.identifier.issn1536-1632
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/193399
dc.description.abstractEnPurpose: Transport across the plasma membrane is a critical step of drug delivery for weakly permeable compounds with intracellular mode of action. The purpose of this study is to demonstrate real-time monitoring of ultrasound (US)-mediated cell-impermeable model drug uptake with fibered confocal fluorescence microscopy (FCFM). Procedures: An in vitro setup was designed to combine a mono-element US transducer, a cell chamber with a monolayer of tumor cells together with SonoVue microbubbles, and a FCFM system. The cell-impermeable intercalating dye, SYTOX Green, was used to monitor US-mediated uptake. Results: The majority of the cell population showed fluorescence signal enhancement 10 s after US onset. The mean rate constant k of signal enhancement was calculated to be 0.23±0.04 min −1. Conclusions: Feasibility of real-time monitoring of US-mediated intracellular delivery by FCFM has been demonstrated. The method allowed quantitative assessment of model drug uptake, holding great promise for further local drug delivery studies.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subject.enDrug delivery
dc.subject.enBiological barrier
dc.subject.enPlasma membrane permeabilization
dc.subject.enUltrasound
dc.subject.enbioeffects
dc.subject.enFibered confocal fluorescence microscopy
dc.subject.enPharmacokinetic parameters
dc.subject.enSYTOX
dc.subject.enGreen
dc.subject.enUS-mediated drug delivery
dc.title.enReal-Time Assessment of Ultrasound-Mediated Drug Delivery Using Fibered Confocal Fluorescence Microscopy
dc.typeArticle de revue
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'image
dc.subject.halInformatique [cs]/Traitement du signal et de l'image
dc.subject.halInformatique [cs]/Imagerie médicale
bordeaux.journalMolecular Imaging and Biology
bordeaux.page3-11
bordeaux.volume15
bordeaux.hal.laboratoriesInstitut de Mathématiques de Bordeaux (IMB) - UMR 5251*
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00994203
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00994203v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Imaging%20and%20Biology&rft.date=2013-02-01&rft.volume=15&rft.issue=1&rft.spage=3-11&rft.epage=3-11&rft.eissn=1536-1632&rft.issn=1536-1632&rft.au=DERIEPPE,%20Marc&YUDINA,%20Anna&LEPETIT-COIFF%C3%89,%20Matthieu&DENIS%20DE%20SENNEVILLE,%20Baudouin&BOS,%20Clemens&rft.genre=article


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