GIRAULT, Mathias; SIANO, Raffaele; LABRY, Claire; LATIMIER, Marie; JAUZEIN, Cécile; BENEYTON, Thomas; BUISSON, Lionel; DEL AMO, Yolanda; BARET, Jean-Christophe

doi:10.1038/s41396-021-00904-2

Centre de Recherche Paul Pascal [CRPP]

SIANO, Raffaele
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]

LABRY, Claire
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]

Langue

Article de revue

Ce document a été publié dans

ISME Journal. 2021-02-10

Résumé en anglais

Adaptation of cell populations to environmental changes is mediated by phenotypic variability at the single-cell level. Enzyme activity is a key factor in cell phenotype and the expression of the alkaline phosphatase activity (APA) is a fundamental phytoplankton strategy for maintaining growth under phosphate-limited conditions. Our aim was to compare the APA among cells and species revived from sediments of the Bay of Brest (Brittany, France), corresponding to a pre-eutrophication period (1940’s) and a beginning of a post-eutrophication period (1990’s) during which phosphate concentrations have undergone substantial variations. Both toxic marine dinoflagellate Alexandrium minutum and the non-toxic dinoflagellate Scrippsiella acuminata were revived from ancient sediments. Using microfluidics, we measured the kinetics of APA at the single-cell level. Our results indicate that all S. acuminata strains had significantly higher APA than A. minutum strains. For both species, the APA in the 1990’s decade was significantly lower than in the 1940’s. For the first time, our results reveal both inter and intraspecific variabilities of dinoflagellate APA and suggest that, at a half-century timescale, two different species of dinoflagellate may have undergone similar adaptative evolution to face environmental changes and acquire ecological advantages.< Réduire

Mots clés en anglais

Alkaline phosphatase activity

single cell

microfluidic

ELF

dinoflagellate

Alexandrium minutum

Scrippsiella acuminata

URI

https://oskar-bordeaux.fr/handle/20.500.12278/188225

DOI

http://dx.doi.org/10.1038/s41396-021-00904-2

Project ANR

Initiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003

Unités de recherche

EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805

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Variable inter and intraspecies alkaline phosphatase activity within single cells of revived dinoflagellates