Afficher la notice abrégée

Variable inter and intraspecies alkaline phosphatase activity within single cells of revived dinoflagellates

dc.rights.licenseopenen_US
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorGIRAULT, Mathias
hal.structure.identifierInstitut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
dc.contributor.authorSIANO, Raffaele
hal.structure.identifierInstitut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
dc.contributor.authorLABRY, Claire
hal.structure.identifierInstitut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
dc.contributor.authorLATIMIER, Marie
hal.structure.identifierInstitut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
dc.contributor.authorJAUZEIN, Cécile
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBENEYTON, Thomas
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBUISSON, Lionel
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDEL AMO, Yolanda
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
hal.structure.identifierInstitut universitaire de France [IUF]
dc.contributor.authorBARET, Jean-Christophe
dc.date.accessioned2024-02-17T09:47:28Z
dc.date.available2024-02-17T09:47:28Z
dc.date.issued2021-02-10
dc.identifier.issn1751-7362en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/188225
dc.description.abstractEnAdaptation of cell populations to environmental changes is mediated by phenotypic variability at the single-cell level. Enzyme activity is a key factor in cell phenotype and the expression of the alkaline phosphatase activity (APA) is a fundamental phytoplankton strategy for maintaining growth under phosphate-limited conditions. Our aim was to compare the APA among cells and species revived from sediments of the Bay of Brest (Brittany, France), corresponding to a pre-eutrophication period (1940’s) and a beginning of a post-eutrophication period (1990’s) during which phosphate concentrations have undergone substantial variations. Both toxic marine dinoflagellate Alexandrium minutum and the non-toxic dinoflagellate Scrippsiella acuminata were revived from ancient sediments. Using microfluidics, we measured the kinetics of APA at the single-cell level. Our results indicate that all S. acuminata strains had significantly higher APA than A. minutum strains. For both species, the APA in the 1990’s decade was significantly lower than in the 1940’s. For the first time, our results reveal both inter and intraspecific variabilities of dinoflagellate APA and suggest that, at a half-century timescale, two different species of dinoflagellate may have undergone similar adaptative evolution to face environmental changes and acquire ecological advantages.
dc.description.sponsorshipInitiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003en_US
dc.language.isoENen_US
dc.subject.enAlkaline phosphatase activity
dc.subject.ensingle cell
dc.subject.enmicrofluidic
dc.subject.enELF
dc.subject.endinoflagellate
dc.subject.enAlexandrium minutum
dc.subject.enScrippsiella acuminata
dc.title.enVariable inter and intraspecies alkaline phosphatase activity within single cells of revived dinoflagellates
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41396-021-00904-2en_US
dc.subject.halSciences de l'environnement/Milieux et Changements globauxen_US
dc.subject.halChimie/Chimie analytiqueen_US
dc.subject.halPhysique [physics]en_US
bordeaux.journalISME Journalen_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamECOBIOCen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-03146965
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ISME%20Journal&rft.date=2021-02-10&rft.eissn=1751-7362&rft.issn=1751-7362&rft.au=GIRAULT,%20Mathias&SIANO,%20Raffaele&LABRY,%20Claire&LATIMIER,%20Marie&JAUZEIN,%20C%C3%A9cile&rft.genre=article


Fichier(s) constituant ce document

Thumbnail
Nom:
GiraultISME2021.pdf
Taille:
2.513Mo
Format:
PDF
Voir/Ouvrir

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée