Plastic is currently used in aquaculture as a material for settlement and magnification of oyster spats. Plastic weathering and fragmentation under natural conditions can lead to the production of micro and nanoparticles and additive leakage, with potential toxic effects on marine life. This study investigates the effects of the exposure to microplastic (MPs) cocktail derived from aged aquaculture material on oyster pediveliger larvae (Crassostrea gigas). The cocktail was made of high-density polyethylene (HDPE), polypropylene (PP) and polyvinyl chloride (PVC). The concentrations tested were 0, 0.1, and 10 mg MP·L−1. During the 7-day fixation phase, pediveliger larvae (17 days) were exposed to the MP cocktail in laboratory-controlled conditions. After exposure, the success of settlement was significantly lower for larvae exposed to 10 mg MP·L−1 (49 ± 0.9%) compared to control ones (61.8 ± 1.6%). No malformations or metamorphosis abnormalities were observed. Growth of pediveliger and spat stages was monitored up to 11 months. During the first twenty-eight days of development, spat growth was significantly lower for the two MPs exposure conditions (0.1 and 10 mg MP·L−1; respectively −51.8% and −44.4%) compared to control group. Subsequently, the previously exposed oysters grew faster than the control condition, resulting in a significantly greater growth (0.1 and 10 mg MP·L−1: +18.3% and +19.7%) than the control group at the end of follow-up. The nearly one-year follow-up highlighted the potential effects of MPs from aquaculture on larvae and spat of C. gigas.Read less <