Cassette recombination dynamics within chromosomal integrons are regulated by toxin-antitoxin systems.
ROCHA, Eduardo P C
Institut Pasteur [Paris] [IP]
Génétique des génomes - Genetics of Genomes [UMR 3525]
< Leer menos
Institut Pasteur [Paris] [IP]
Génétique des génomes - Genetics of Genomes [UMR 3525]
Idioma
EN
Article de revue
Este ítem está publicado en
Science Advances. 2024-01-12, vol. 10, n° 2, p. eadj3498
Resumen en inglés
Integrons are adaptive bacterial devices that rearrange promoter-less gene cassettes into variable ordered arrays under stress conditions, thereby sampling combinatorial phenotypic diversity. Chromosomal integrons often ...Leer más >
Integrons are adaptive bacterial devices that rearrange promoter-less gene cassettes into variable ordered arrays under stress conditions, thereby sampling combinatorial phenotypic diversity. Chromosomal integrons often carry hundreds of silent gene cassettes, with integrase-mediated recombination leading to rampant DNA excision and integration, posing a potential threat to genome integrity. How this activity is regulated and controlled, particularly through selective pressures, to maintain such large cassette arrays is unknown. Here, we show a key role of promoter-containing toxin-antitoxin (TA) cassettes as systems that kill the cell when the overall cassette excision rate is too high. These results highlight the importance of TA cassettes regulating the cassette recombination dynamics and provide insight into the evolution and success of integrons in bacterial genomes.< Leer menos
Palabras clave en inglés
Integrons
Toxin-Antitoxin Systems
Bacteria
Genome
Bacterial
Recombination
Genetic
Centros de investigación