Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension.
| dc.rights.license | open | en_US |
| dc.contributor.author | MARIE, Lea | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | RAPISARDA, Chiara | |
| hal.structure.identifier | Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM] | |
| dc.contributor.author | MORALES, Violette | |
| hal.structure.identifier | Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM] | |
| dc.contributor.author | BERGE, Mathieu | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | PERRY, Thomas | |
| hal.structure.identifier | Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM] | |
| dc.contributor.author | SOULET, Anne-Lise | |
| dc.contributor.author | GRUGET, Clemence | |
| hal.structure.identifier | VIB-VUB Center for Structural Biology [Bruxelles] | |
| dc.contributor.author | REMAUT, Han | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | FRONZES, Remi | |
| hal.structure.identifier | Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM] | |
| dc.contributor.author | POLARD, Patrice | |
| dc.date.accessioned | 2023-11-20T14:47:45Z | |
| dc.date.available | 2023-11-20T14:47:45Z | |
| dc.date.issued | 2017-05-31 | |
| dc.identifier.issn | 2041-1723 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184853 | |
| dc.description.abstractEn | Homologous recombination (HR) is a central process of genome biology driven by a conserved recombinase, which catalyses the pairing of single-stranded DNA (ssDNA) with double-stranded DNA to generate a D-loop intermediate. Bacterial RadA is a conserved HR effector acting with RecA recombinase to promote ssDNA integration. The mechanism of this RadA-mediated assistance to RecA is unknown. Here, we report functional and structural analyses of RadA from the human pathogen Streptococcus pneumoniae. RadA is found to facilitate RecA-driven ssDNA recombination over long genomic distances during natural transformation. RadA is revealed as a hexameric DnaB-type helicase, which interacts with RecA to promote orientated unwinding of branched DNA molecules mimicking D-loop boundaries. These findings support a model of DNA branch migration in HR, relying on RecA-mediated loading of RadA hexamers on each strand of the recipient dsDNA in the D-loop, from which they migrate divergently to facilitate incorporation of invading ssDNA. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Bacterial Proteins | |
| dc.subject.en | Crystallography | |
| dc.subject.en | X-Ray | |
| dc.subject.en | DNA Helicases | |
| dc.subject.en | DNA | |
| dc.subject.en | Single-Stranded | |
| dc.subject.en | DNA-Binding Proteins | |
| dc.subject.en | DnaB Helicases | |
| dc.subject.en | Homologous Recombination | |
| dc.subject.en | Mutagenesis | |
| dc.subject.en | Site-Directed | |
| dc.subject.en | Protein Domains | |
| dc.subject.en | Protein Structure | |
| dc.subject.en | Quaternary | |
| dc.subject.en | Rec A Recombinases | |
| dc.subject.en | Recombination | |
| dc.subject.en | Genetic | |
| dc.subject.en | Streptococcus pneumoniae | |
| dc.subject.en | Two-Hybrid System Techniques | |
| dc.title.en | Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension. | |
| dc.title.alternative | Nat Commun | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1038/ncomms15638 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 28561029 | en_US |
| bordeaux.journal | Nature Communications | en_US |
| bordeaux.page | 15638 | en_US |
| bordeaux.volume | 8 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04296073 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-11-20T14:47:48Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Communications&rft.date=2017-05-31&rft.volume=8&rft.spage=15638&rft.epage=15638&rft.eissn=2041-1723&rft.issn=2041-1723&rft.au=MARIE,%20Lea&RAPISARDA,%20Chiara&MORALES,%20Violette&BERGE,%20Mathieu&PERRY,%20Thomas&rft.genre=article |
