CALMELS, Christina; VENTURA, Michel; AKNIN, Cindy; METIFIOT, Mathieu; ANDREOLA, Marie-Line

CALMELS, Christina
Microbiologie Fondamentale et Pathogénicité [MFP]

VENTURA, Michel
Microbiologie Fondamentale et Pathogénicité [MFP]

AKNIN, Cindy
Microbiologie Fondamentale et Pathogénicité [MFP]

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Article de revue

Este ítem está publicado en

Scientific Reports. 2017-06-02, vol. 7, n° 1, p. 2697

Resumen en inglés

Mosquito- and tick-borne pathogens including Chikungunya, Dengue, Japanese encephalitis, West Nile, Yellow fever and Zika virus, represent a new economic and public health challenge. In the absence of effective vaccines and specific therapies, only supportive regimens are administrated for most of these infections. Thus, the development of a targeted therapy is mandatory to stop the rapid progression of these pathogens and preoccupant associated burdens such as Guillain-Barre syndrome, microcephaly. For this, it is essential to develop biochemical tools to help study and target key viral enzymes involved in replication such as helicase complexes, methyl-transferases and RNA-dependent RNA polymerases. Here, we show that a highly purified ZIKV polymerase domain is active in vitro. Importantly, we show that this isolated domain is capable of de novo synthesis of the viral genome and efficient elongation without terminal nucleotide transferase activity. Altogether, this isolated polymerase domain will be a precious tool to screen and optimize specific nucleoside and non-nucleoside inhibitors to fight against Zika infections.< Leer menos

Palabras clave en inglés

Catalysis

Humans

Models

Molecular

Protein Binding

Protein Conformation

Protein Domains

Protein Interaction Domains and Motifs

RNA

Viral

RNA-Dependent RNA Polymerase

Transcription

Genetic

Virus Replication

Zika Virus

Zika Virus Infection

URI

https://oskar-bordeaux.fr/handle/20.500.12278/184677

Centros de investigación

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

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De novo RNA synthesis catalyzed by the Zika Virus RNA polymerase domain.

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Resumen en inglés

Palabras clave en inglés

URI

Centros de investigación