NOUBHANI, A.; BEGU, Dominique; CHAIGNEPAIN, S.; MOHA OU MAATI, H.; BORSOTTO, M.; DUPUY, J; LANGLOIS D'ESTAINTOT, B.; SANTARELLI, X.; HEURTEAUX, C.; GALLOIS, B.; HUGUES, M.

doi:10.1016/j.bbrep.2015.10.003

BEGU, Dominique
Microbiologie Fondamentale et Pathogénicité [MFP]

CHAIGNEPAIN, S.
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

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2015-10-09, vol. 4, p. 299 - 305

Résumé en anglais

Mapacalcine is a small homodimeric protein of 19 kDa with 9 disulfide bridges extracted from the Cliona vastifica sponge (Red Sea). It selectively blocks a calcium current insensitive to most calcium blockers. Specific receptors for mapacalcine have been described in a variety of tissues such as brain, smooth muscle, liver, and kidney. Previous works achieved on hepatocytes and nervous cells demonstrated that this protein selectively blocks a calcium influx triggered by an ischemia/reperfusion (I/R) shock and efficiently protects cells from death after I/R. The aim of this work was to produce the recombinant mapacalcine in the yeast Pichia pastoris. Mass spectrometry, light scattering analysis and biological characterization demonstrated that the recombinant mapacalcine obtained was a monomeric form with 4 disulfide bridges which retains the biological activity of the natural protein.< Réduire

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https://oskar-bordeaux.fr/handle/20.500.12278/183388

DOI

http://dx.doi.org/10.1016/j.bbrep.2015.10.003

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

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Production, in Pichia pastoris, of a recombinant monomeric mapacalcine, a protein with anti-ischemic properties

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