HÜSING, Svenja; VAN LOOK, Ulf; GUSE, Alina; GÁLVEZ, Eric; CHARPENTIER, Emmanuelle; BLAIR, David; ERHARDT, Marc; RENAULT, Thibaud

doi:10.1101/2020.11.25.397760

GUSE, Alina
Humboldt-Universität zu Berlin = Humboldt University of Berlin = Université Humboldt de Berlin [HU Berlin]

Langue

Document de travail - Pré-publication

Résumé en anglais

Type-III secretion systems (T3SSs) of the bacterial flagellum and the evolutionarily related injectisome are capable of translocating proteins with a remarkable speed of several thousand amino acids per second. Here, we investigated how T3SSs are able to transport proteins at such a high rate while preventing the leakage of small molecules. Our mutational and evolutionary analyses demonstrate that an ensemble of conserved methionine residues at the cytoplasmic side of the T3SS channel create a deformable gasket (M-gasket) around fast-moving substrates undergoing export. The unique physicochemical features of the M-gasket are crucial to preserve the membrane barrier, to accommodate local conformational changes during active secretion, and to maintain stability of the secretion pore in cooperation with a plug domain (R-plug) and a network of salt-bridges. The conservation of the M-gasket, R-plug, and salt-bridge network suggests a universal mechanism by which the membrane integrity is maintained during high-speed protein translocation in all T3SSs.< Réduire

Mots clés en anglais

type-III secretion

flagellum

Salmonella

membrane barrier

protein secretion

URI

https://oskar-bordeaux.fr/handle/20.500.12278/182397

DOI

http://dx.doi.org/10.1101/2020.11.25.397760

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

Voir/Ouvrir

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Controlling membrane barrier during bacterial type-III protein secretion

Langue

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche