MHAYA, Amel; BÉGU, Dominique; TOUNSI, Slim; ARPIN, Corinne

doi:10.1128/AAC.00128-20

Microbiologie Fondamentale et Pathogénicité [MFP]

BÉGU, Dominique
Microbiologie Fondamentale et Pathogénicité [MFP]

TOUNSI, Slim

Langue

Article de revue

Ce document a été publié dans

Antimicrobial Agents and Chemotherapy. 2020, vol. 64, n° 6, p. e00128-20

Résumé en anglais

Multidrug-resistant strains belonging to the Enterobacter cloacae complex (ECC) group, and especially those belonging to clusters C-III, C-IV, and C-VIII, have increasingly emerged as a leading cause of health care-associated infections, with colistin used as one of the last lines of treatment. However, colistin-resistant ECC strains have emerged. The aim of this study was to prove that MgrB, the negative regulator of the PhoP/PhoQ two-component regulatory system, is involved in colistin resistance in ECC of cluster C-VIII, formerly referred to as Enterobacter hor-maechei subsp. steigerwaltii. An in vitro mutant (Eh22-Mut) was selected from a clinical isolate of Eh22. The sequencing analysis of its mgrB gene showed the presence of one nucleotide deletion leading to the formation of a truncated protein of six instead of 47 amino acids. The wild-type mgrB gene from Eh22 and that of a clinical strain of Klebsiella pneumoniae used as controls were cloned, and the corresponding recombinant plasmids were used for complementation assays. The results showed a fully restored susceptibility to colistin and confirmed for the first time that mgrB gene expression plays a key role in acquired resistance to colistin in ECC strains.< Réduire

Mots clés en anglais

Enterobacter cloacae complex

Enterobacter hormaechei subsp steigerwaltii

colistin resistance

MgrB

PhoPQ regulatory system

URI

https://oskar-bordeaux.fr/handle/20.500.12278/182391

DOI

http://dx.doi.org/10.1128/AAC.00128-20

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

Voir/Ouvrir

Métadonnées

Partager cette publication !

Licence d’utilisation du document

MgrB Inactivation Is Responsible for Acquired Resistance to Colistin in Enterobacter hormaechei subsp. steigerwaltii

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche