LAURIAN, Romain; RAVENT, Jade; DEMENTHON, Karine; LEMAIRE, Marc; SOULARD, Alexandre; COTTON, Pascale

doi:10.3390/microorganisms9040848

Microbiologie Fondamentale et Pathogénicité [MFP]

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Ce document a été publié dans

Microorganisms. 2021, vol. 9, n° 4, p. 848

Résumé en anglais

Survival of the pathogenic yeast Candida albicans depends upon assimilation of fermentable and non-fermentable carbon sources detected in host microenvironments. Among the various carbon sources encountered in a human body, glucose is the primary source of energy. Its effective detection, metabolism and prioritization via glucose repression are primordial for the metabolic adaptation of the pathogen. In C. albicans, glucose phosphorylation is mainly performed by the hexokinase 2 (CaHxk2). In addition, in the presence of glucose, CaHxK2 migrates in the nucleus and contributes to the glucose repression signaling pathway. Based on the known dual function of the Saccharomyces cerevisiae hexokinase 2 (ScHxk2), we intended to explore the impact of both enzymatic and regulatory functions of CaHxk2 on virulence, using a site-directed mutagenesis approach. We show that the conserved aspartate residue at position 210, implicated in the interaction with glucose, is essential for enzymatic and glucose repression functions but also for filamentation and virulence in macrophages. Point mutations and deletion into the N-terminal region known to specifically affect glucose repression in ScHxk2 proved to be ineffective in CaHxk2. These results clearly show that enzymatic and regulatory functions of the hexokinase 2 cannot be unlinked in C. albicans.< Réduire

Mots clés en anglais

Saccharomyces cerevisiae

hexokinase 2

glucose repression

hexose kinase activity

hyphal transition

virulence

URI

https://oskar-bordeaux.fr/handle/20.500.12278/182264

DOI

http://dx.doi.org/10.3390/microorganisms9040848

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

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Candida albicans Hexokinase 2 Challenges the Saccharomyces cerevisiae Moonlight Protein Model

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