MONIC, S.G.; LAMY, A.; THONNUS, M.; BIZARRA-REBELO, T.; BRINGAUD, F.; SMITH, T.; FIGUEIREDO, L.; RIVIÈRE, L.

doi:10.1038/s41598-022-08546-w

MONIC, S.G.
Microbiologie Fondamentale et Pathogénicité [MFP]

LAMY, A.

THONNUS, M.
Microbiologie Fondamentale et Pathogénicité [MFP]

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Article de revue

Ce document a été publié dans

Scientific Reports. 2022-12, vol. 12, n° 1, p. 4766

Résumé en anglais

Abstract Phospholipases are esterases involved in lipid catabolism. In pathogenic micro-organisms (bacteria, fungi, parasites) they often play a critical role in virulence and pathogenicity. A few phospholipases (PL) have been characterised so far at the gene and protein level in unicellular parasites including African trypanosomes (AT). They could play a role in different processes such as host–pathogen interaction, antigenic variation, intermediary metabolism. By mining the genome database of AT we found putative new phospholipase candidate genes and here we provided biochemical evidence that one of these has lipolytic activity. This protein has a unique non-canonical glycosome targeting signal responsible for its dual localisation in the cytosol and the peroxisomes-related organelles named glycosomes. We also show that this new phospholipase is excreted by these pathogens and that antibodies directed against this protein are generated during an experimental infection with T. brucei gambiense , a subspecies responsible for infection in humans. This feature makes this protein a possible tool for diagnosis.< Réduire

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https://oskar-bordeaux.fr/handle/20.500.12278/182079

DOI

http://dx.doi.org/10.1038/s41598-022-08546-w

Project ANR

Interactions métaboliques entre les adipocytes et les trypanosomes, un nouveau paradigme pour les trypanosomoses - ANR-19-CE15-0004
Voies métaboliques glycosomales non glycolytiques: nouvelles fonctions pour le développement et la virulence des trypanosomes - ANR-15-CE15-0025
Alliance française contre les maladies parasitaires - ANR-11-LABX-0024

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

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A novel lipase with dual localisation in Trypanosoma brucei

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Résumé en anglais

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DOI

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