Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry
Langue
en
Article de revue
Ce document a été publié dans
Optics Express. 2015-06-15, vol. 23, n° 12, p. 16383-16406
Optical Society of America - OSA Publishing
Résumé en anglais
We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system ...Lire la suite >
We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system combines quantitative phase images with varying polarization excitation to create retardance images. This technique is compatible with living samples and gives information about the local retardance and structure of their anisotropic components. We applied our approach to collagen fibers leading to a birefringence value of (3.4 +/- 0.3) . 10(-3) and to living cells, showing that cytoskeleton can be imaged label-free.< Réduire
Mots clés en anglais
OPTICAL COHERENCE TOMOGRAPHY
BIREFRINGENCE
COLLAGEN
DIGITAL HOLOGRAPHIC MICROSCOPY
POLARIZED PHASE MICROSCOPY
MATRICES
RESOLUTION
TRANSPARENT
LIVING CELLS
REFRACTIVE-INDEX
Origine
Importé de halUnités de recherche