Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry
Language
en
Article de revue
This item was published in
Optics Express. 2015-06-15, vol. 23, n° 12, p. 16383-16406
Optical Society of America - OSA Publishing
English Abstract
We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system ...Read more >
We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system combines quantitative phase images with varying polarization excitation to create retardance images. This technique is compatible with living samples and gives information about the local retardance and structure of their anisotropic components. We applied our approach to collagen fibers leading to a birefringence value of (3.4 +/- 0.3) . 10(-3) and to living cells, showing that cytoskeleton can be imaged label-free.Read less <
English Keywords
OPTICAL COHERENCE TOMOGRAPHY
BIREFRINGENCE
COLLAGEN
DIGITAL HOLOGRAPHIC MICROSCOPY
POLARIZED PHASE MICROSCOPY
MATRICES
RESOLUTION
TRANSPARENT
LIVING CELLS
REFRACTIVE-INDEX
Origin
Hal imported