Innovative molecular-based fluorescent nanoparticles for multicolor single particle tracking in cells
Langue
en
Article de revue
Ce document a été publié dans
Journal of Physics D: Applied Physics. 2016, vol. 49, p. 84002 - 84002
IOP Publishing
Résumé en anglais
Based on an original molecular-based design we present bright and photostable fluorescent organic nanoparticles (FONs) with an excellent colloidal stability in various aqueous environments. Near-infrared emitting and green ...Lire la suite >
Based on an original molecular-based design we present bright and photostable fluorescent organic nanoparticles (FONs) with an excellent colloidal stability in various aqueous environments. Near-infrared emitting and green emitting FONs were prepared using a simple and robust protocol. Both types of FONs could be simultaneously imaged at the single-particle level in solution as well as in biological environments using a monochromatic excitation and a dual-color fluorescence microscope. No evidence of acute cytotoxicity was found upon incubation of live cells with mixed solutions of FONs, and both types of nanoparticles were found internalized in the cells where their movements could be simultaneously tracked at video-rate up to minutes. These fluorescent FONs open a novel non-toxic alternative to existing nanoparticles for imaging biological structures, compatible with live-cell experiments and specially fitted for multicolor single particle tracking.< Réduire
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