WINCKLER, Pascale; LARTIGUE, Lydia; GIANNONE, Gregory; DE GIORGI, Francesca; ICHAS, François; SIBARITA, Jean-Baptiste; LOUNIS, Brahim; COGNET, Laurent

doi:10.1038/srep02387

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

WINCKLER, Pascale
Laboratoire Photonique, Numérique et Nanosciences [LP2N]

LARTIGUE, Lydia
Plateforme de génétique moléculaire des cancers d'Aquitaine

GIANNONE, Gregory

Langue

Article de revue

Ce document a été publié dans

Scientific Reports. 2013-08-08, vol. 3, p. 2387

Nature Publishing Group

Résumé en anglais

Molecular interactions are key to many chemical and biological processes like protein function. In many signaling processes they occur in sub-cellular areas displaying nanoscale organizations and involving molecular assemblies. The nanometric dimensions and the dynamic nature of the interactions make their investigations complex in live cells. While super-resolution fluorescence microscopies offer live-cell molecular imaging with sub-wavelength resolutions, they lack specificity for distinguishing interacting molecule populations. Here we combine super-resolution microscopy and single-molecule Förster Resonance Energy Transfer (FRET) to identify dimers of receptors induced by ligand binding and provide super-resolved images of their membrane distribution in live cells. By developing a two-color universal-Point-Accumulation-In-the-Nanoscale-Topography (uPAINT) method, dimers of epidermal growth factor receptors (EGFR) activated by EGF are studied at ultra-high densities, revealing preferential cell-edge sub-localization. This methodology which is specifically devoted to the study of molecules in interaction, may find other applications in biological systems where understanding of molecular organization is crucial.< Réduire

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Identification and super-resolution imaging of ligand-activated receptor dimers in live cells

Langue

Ce document a été publié dans

Résumé en anglais

URI

DOI

Origine

Unités de recherche