Identification and super-resolution imaging of ligand-activated receptor dimers in live cells
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | WINCKLER, Pascale | |
| hal.structure.identifier | Plateforme de génétique moléculaire des cancers d'Aquitaine | |
| dc.contributor.author | LARTIGUE, Lydia | |
| dc.contributor.author | GIANNONE, Gregory | |
| hal.structure.identifier | Fluofarma | |
| dc.contributor.author | DE GIORGI, Francesca | |
| hal.structure.identifier | Fluofarma | |
| dc.contributor.author | ICHAS, François | |
| dc.contributor.author | SIBARITA, Jean-Baptiste | |
| hal.structure.identifier | lp2n-04,lp2n-12 | |
| dc.contributor.author | LOUNIS, Brahim | |
| hal.structure.identifier | lp2n-04,lp2n-12 | |
| dc.contributor.author | COGNET, Laurent | |
| dc.date.accessioned | 2023-05-12T10:21:23Z | |
| dc.date.available | 2023-05-12T10:21:23Z | |
| dc.date.issued | 2013-08-08 | |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/181107 | |
| dc.description.abstractEn | Molecular interactions are key to many chemical and biological processes like protein function. In many signaling processes they occur in sub-cellular areas displaying nanoscale organizations and involving molecular assemblies. The nanometric dimensions and the dynamic nature of the interactions make their investigations complex in live cells. While super-resolution fluorescence microscopies offer live-cell molecular imaging with sub-wavelength resolutions, they lack specificity for distinguishing interacting molecule populations. Here we combine super-resolution microscopy and single-molecule Förster Resonance Energy Transfer (FRET) to identify dimers of receptors induced by ligand binding and provide super-resolved images of their membrane distribution in live cells. By developing a two-color universal-Point-Accumulation-In-the-Nanoscale-Topography (uPAINT) method, dimers of epidermal growth factor receptors (EGFR) activated by EGF are studied at ultra-high densities, revealing preferential cell-edge sub-localization. This methodology which is specifically devoted to the study of molecules in interaction, may find other applications in biological systems where understanding of molecular organization is crucial. | |
| dc.language.iso | en | |
| dc.publisher | Nature Publishing Group | |
| dc.title.en | Identification and super-resolution imaging of ligand-activated receptor dimers in live cells | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1038/srep02387 | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
| dc.identifier.arxiv | 1312.0892 | |
| bordeaux.journal | Scientific Reports | |
| bordeaux.page | 2387 | |
| bordeaux.volume | 3 | |
| bordeaux.hal.laboratories | Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298 | * |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | CNRS | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-00909281 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00909281v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2013-08-08&rft.volume=3&rft.spage=2387&rft.epage=2387&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=WINCKLER,%20Pascale&LARTIGUE,%20Lydia&GIANNONE,%20Gregory&DE%20GIORGI,%20Francesca&ICHAS,%20Fran%C3%A7ois&rft.genre=article |
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