YOO, Sung Hyun; BURATTO, Jeremie; ROY, Arup; MORVAN, Estelle; PASCO, Morgane; PULKA-ZIACH, Karolina; LOMBARDO, Caterina M.; ROSU, Frederic; GABELICA, Valerie; MACKERETH, Cameron D.; COLLIE, Gavin W.; GUICHARD, Gilles

doi:10.1021/jacs.2c05234

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

BURATTO, Jeremie
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

ROY, Arup
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

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Article de revue

Ce document a été publié dans

Journal of the American Chemical Society. 2022-08-23, vol. 144, n° 35, p. 15988-15998

Résumé en anglais

Amphipathic water-soluble helices formed from synthetic peptides or foldamers are promising building blocks for the creation of self-assembled architectures with non-natural shapes and functions. While rationally designed artificial quaternary structures such as helix bundles have been shown to contain preformed cavities suitable for guest binding, there are no examples of adaptive binding of guest molecules by such assemblies in aqueous conditions. We have previously reported a foldamer 6-helix bundle that contains an internal nonpolar cavity able to bind primary alcohols as guest molecules. Here, we show that this 6-helix bundle can also interact with larger, more complex guests such as n-alkyl glycosides. X-ray diffraction analysis of co-crystals using a diverse set of guests together with solution and gas-phase studies reveals an adaptive binding mode whereby the apo form of the 6-helix bundle undergoes substantial conformational change to accommodate the hydrocarbon chain in a manner reminiscent of glycolipid transfer proteins in which the cavity forms upon lipid uptake. The dynamic nature of the self-assembling and molecular recognition processes reported here marks a step forward in the design of functional proteomimetic molecular assemblies.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/173076

DOI

http://dx.doi.org/10.1021/jacs.2c05234

Projet Européen

Bioinspired Nanostructures by Self-assembly of Amphiphilic Non-peptide Helical Foldamers in Aqueous Environment

Project ANR

Mimétisme et ingénierie d'enzymes artificielles à l'aide de foldamères

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248

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Adaptive Binding of Alkyl Glycosides by Nonpeptidic Helix Bundles in Water: Toward Artificial Glycolipid Binding Proteins