KONG, Junhua; GARCIA, Virginie; ZEHRAOUI, Enric; STAMMITTI, Linda; GODINAUD, Ghislaine; RENAUD, Christel; MAURY, Stéphane; DELAUNAY, Alain; CLUZET, Stephanie; LECOURIEUX, Fatma; LECOURIEUX, David; TEYSSIER, Emeline; GALLUSCI, Philippe

doi:10.3390/genes13071256

KONG, Junhua
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

GARCIA, Virginie
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

ZEHRAOUI, Enric
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

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Article de revue

Ce document a été publié dans

Genes. 2022-07-15, vol. 13, n° 7, p. 1256

Résumé en anglais

Through its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier cell suspensions. In this model, zebularine increased anthocyanin accumulation in the light, and induced its production in the dark. To unravel the underlying mechanisms, cell transcriptome, metabolic content, and DNA methylation were analyzed. The up-regulation of stress-related genes, as well as a decrease in cell viability, revealed that zebularine affected cell integrity. Concomitantly, the global DNA methylation level was only slightly decreased in the light and not modified in the dark. However, locus-specific analyses demonstrated a decrease in DNA methylation at a few selected loci, including a CACTA DNA transposon and a small region upstream from the UFGT gene, coding for the UDP glucose:flavonoid-3-O-glucosyltransferase, known to be critical for anthocyanin biosynthesis. Moreover, this decrease was correlated with an increase in UFGT expression and in anthocyanin content. In conclusion, our data suggest that UFGT expression could be regulated through DNA methylation in Gamay Teinturier, although the functional link between changes in DNA methylation and UFGT transcription still needs to be demonstrated.< Réduire

Mots clés en anglais

DNA methylation

Zebularine

Anthocyanin

URI

https://oskar-bordeaux.fr/handle/20.500.12278/172010

DOI

http://dx.doi.org/10.3390/genes13071256

Lien vers les données de la recherche

https://mdpi-res.com/d_attachment/genes/genes-13-01256/article_deploy/genes-13-01256-s001.zip?version=1657884457
https://www.ebi.ac.uk/ena/browser/view/PRJEB54585

Unités de recherche

Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287

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Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells