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Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
Idioma
EN
Article de revue
Este ítem está publicado en
Frontiers in Plant Science. 2022-10-25, vol. 13
Resumen en inglés
Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later ...Leer más >
Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thaliana cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and
enzymatic fingerprinting approaches. Relative to the rest of the cell wall,
specific patterns were observed in the PD fraction. Most xyloglucans,
although possibly not abundant as a group, were fucosylated.
Homogalacturonans displayed short methylated stretches while
rhamnogalacturonan I species were remarkably abundant. Ful l
rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD.< Leer menos
Palabras clave en inglés
plasmodesmata
cell wall
Arabidopsis thaliana
enzymatic fingerprinting
xyloglucans
homogalacturonans
rhamnogalacturonan I
rhamnogalacturonan II
Proyecto europeo
The function of membrane tethering in plant intercellular communication
Proyecto ANR
Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay - ANR-17-EURE-0007
Centros de investigación