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dc.rights.licenseopenen_US
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorPATERLINI, A.
dc.contributor.authorSECHET, J.
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorIMMEL, F.
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorGRISON, Magali
dc.contributor.authorPILARD, S.
dc.contributor.authorPELLOUX, J.
dc.contributor.authorMOUILLE, G.
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBAYER, Emmanuelle
dc.contributor.authorVOXEUR, A.
dc.date.accessioned2023-01-26T10:57:23Z
dc.date.available2023-01-26T10:57:23Z
dc.date.issued2022-10-25
dc.identifier.issn1664-462Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/171803
dc.description.abstractEnPlasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thaliana cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and enzymatic fingerprinting approaches. Relative to the rest of the cell wall, specific patterns were observed in the PD fraction. Most xyloglucans, although possibly not abundant as a group, were fucosylated. Homogalacturonans displayed short methylated stretches while rhamnogalacturonan I species were remarkably abundant. Ful l rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD.
dc.description.sponsorshipEcole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay - ANR-17-EURE-0007en_US
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enplasmodesmata
dc.subject.encell wall
dc.subject.enArabidopsis thaliana
dc.subject.enenzymatic fingerprinting
dc.subject.enxyloglucans
dc.subject.enhomogalacturonans
dc.subject.enrhamnogalacturonan I
dc.subject.enrhamnogalacturonan II
dc.title.enEnzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
dc.title.alternativeFront. Plant. Sci.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3389/fpls.2022.1020506en_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaireen_US
dc.description.sponsorshipEuropeThe function of membrane tethering in plant intercellular communicationen_US
bordeaux.journalFrontiers in Plant Scienceen_US
bordeaux.volume13en_US
bordeaux.hal.laboratoriesLaboratoire de Biogenèse Membranaire (LBM) - UMR 5200en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Plant%20Science&rft.date=2022-10-25&rft.volume=13&rft.eissn=1664-462X&rft.issn=1664-462X&rft.au=PATERLINI,%20A.&SECHET,%20J.&IMMEL,%20F.&GRISON,%20Magali&PILARD,%20S.&rft.genre=article


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