DEGACHE, Amelie; POULLETIER DE GANNES, Florence; GARENNE, Andre; RENOM, Remy; PERCHERANCIER, Yann; LAGROYE, Isabelle; BERNUS, Olivier; LEWIS, Noelle

doi:10.1088/2057-1976/ac12e1

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DEGACHE, Amelie
Laboratoire de l'intégration, du matériau au système [IMS]

POULLETIER DE GANNES, Florence
Laboratoire de l'intégration, du matériau au système [IMS]

GARENNE, Andre

Institut des Maladies Neurodégénératives [Bordeaux] [IMN]

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Biomedical Physics & Engineering Express. 2022-07-07, vol. 8, n° 5, p. 055007

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Abstract Cardiac arrhythmias represent about 50% of the cardiovascular diseases which are the first cause of mortality in the world. Implantable medical devices play a major role for treating these arrhythmias. Nevertheless the leads induce an unwanted biological phenomenon called fibrosis. This phenomenon begins at a cellular level and is effective at a macroscopic scale causing tissue remodelling with a local modification of the active cardiac tissue. Fibrosis mechanism is complex but at the cellular level, it mainly consists in cardiac fibroblasts activation and differentiation into myofibroblasts. We developed a simplified in vitro model of cardiac fibrosis, with human cardiac fibroblasts whom differentiation into myofibroblasts was promoted with TGF-β1. Our study addresses an unreported impedance-based method for real-time monitoring of in vitro cardiac fibrosis. The objective was to study whether the differentiation of cardiac fibroblasts in myofibroblasts had a specific signature on the cell index, an impedance-based feature measured by the xCELLigence system. Primary human cardiac fibroblasts were cultured along 6 days, with or without laminin coating, to study the role of this adhesion protein in cultures long-term maintenance. The cultures were characterized in the presence or absence of TGF-β1 and we obtained a significant cell index signature specific to the human cardiac fibroblasts differentiation.< Réduire

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In vitro differentiation of human cardiac fibroblasts into myofibroblasts: characterization using electrical impedance

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