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In vitro differentiation of human cardiac fibroblasts into myofibroblasts: characterization using electrical impedance

dc.rights.licenseopenen_US
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorDEGACHE, Amelie
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorPOULLETIER DE GANNES, Florence
hal.structure.identifierInstitut des Maladies Neurodégénératives [Bordeaux] [IMN]
dc.contributor.authorGARENNE, Andre
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorRENOM, Remy
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorPERCHERANCIER, Yann
IDREF: 075934140
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorLAGROYE, Isabelle
dc.contributor.authorBERNUS, Olivier
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorLEWIS, Noelle
IDREF: 156821346
dc.date.accessioned2022-11-08T12:38:05Z
dc.date.available2022-11-08T12:38:05Z
dc.date.issued2022-07-07
dc.identifier.issn2057-1976en_US
dc.identifier.urioai:crossref.org:10.1088/2057-1976/ac12e1
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/170225
dc.description.abstractEnAbstract Cardiac arrhythmias represent about 50% of the cardiovascular diseases which are the first cause of mortality in the world. Implantable medical devices play a major role for treating these arrhythmias. Nevertheless the leads induce an unwanted biological phenomenon called fibrosis. This phenomenon begins at a cellular level and is effective at a macroscopic scale causing tissue remodelling with a local modification of the active cardiac tissue. Fibrosis mechanism is complex but at the cellular level, it mainly consists in cardiac fibroblasts activation and differentiation into myofibroblasts. We developed a simplified in vitro model of cardiac fibrosis, with human cardiac fibroblasts whom differentiation into myofibroblasts was promoted with TGF-β1. Our study addresses an unreported impedance-based method for real-time monitoring of in vitro cardiac fibrosis. The objective was to study whether the differentiation of cardiac fibroblasts in myofibroblasts had a specific signature on the cell index, an impedance-based feature measured by the xCELLigence system. Primary human cardiac fibroblasts were cultured along 6 days, with or without laminin coating, to study the role of this adhesion protein in cultures long-term maintenance. The cultures were characterized in the presence or absence of TGF-β1 and we obtained a significant cell index signature specific to the human cardiac fibroblasts differentiation.
dc.language.isoENen_US
dc.sourcecrossref
dc.title.enIn vitro differentiation of human cardiac fibroblasts into myofibroblasts: characterization using electrical impedance
dc.typeArticle de revueen_US
dc.identifier.doi10.1088/2057-1976/ac12e1en_US
dc.subject.halSciences de l'ingénieur [physics]en_US
bordeaux.journalBiomedical Physics & Engineering Expressen_US
bordeaux.page055007en_US
bordeaux.volume8en_US
bordeaux.hal.laboratoriesLaboratoire d’Intégration du Matériau au Système (IMS) - UMR 5218en_US
bordeaux.issue5en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-03843814
hal.version1
hal.date.transferred2022-11-08T12:38:28Z
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomedical%20Physics%20&%20Engineering%20Express&rft.date=2022-07-07&rft.volume=8&rft.issue=5&rft.spage=055007&rft.epage=055007&rft.eissn=2057-1976&rft.issn=2057-1976&rft.au=DEGACHE,%20Amelie&POULLETIER%20DE%20GANNES,%20Florence&GARENNE,%20Andre&RENOM,%20Remy&PERCHERANCIER,%20Yann&rft.genre=article


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