Untargeted Analysis of Semipolar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation
Langue
en
Chapitre d'ouvrage
Ce document a été publié dans
Plant Metabolomics, Plant Metabolomics. 2018-05-15, vol. 1778, p. 101-124
Springer Science + Business Media B.V.
Résumé en anglais
The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation ...Lire la suite >
The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography-mass spectrometry (LC-MS) and targeted analysis of fatty acids by gas chromatography-mass spectrometry (GC-MS) or gas chromatography with flame ionization detector (GC-FID) are described.< Réduire
Mots clés en anglais
Fatty acids
Flax seeds
Plant cultivation
Sample harvest
Sample preparation
Specialized compounds
Tomato
Origine
Importé de halUnités de recherche