Untargeted Analysis of Semipolar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation
Idioma
en
Chapitre d'ouvrage
Este ítem está publicado en
Plant Metabolomics, Plant Metabolomics. 2018-05-15, vol. 1778, p. 101-124
Springer Science + Business Media B.V.
Resumen en inglés
The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation ...Leer más >
The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography-mass spectrometry (LC-MS) and targeted analysis of fatty acids by gas chromatography-mass spectrometry (GC-MS) or gas chromatography with flame ionization detector (GC-FID) are described.< Leer menos
Palabras clave en inglés
Fatty acids
Flax seeds
Plant cultivation
Sample harvest
Sample preparation
Specialized compounds
Tomato
Orígen
Importado de HalCentros de investigación