A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing.
NATRAN, Annelore
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
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Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
NATRAN, Annelore
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
VERCRUYSSE, Jasmien
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
GONZALEZ, Nathalie
Biologie du fruit et pathologie [BFP]
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Biologie du fruit et pathologie [BFP]
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
DUBOIS, Marieke
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
INZÉ, Dirk
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
< Leer menos
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Idioma
en
Article de revue
Este ítem está publicado en
Nature Plants. 2019, vol. 5, n° 5, p. 539-550
Nature Publishing Group
Resumen en inglés
Post-transcriptional gene silencing (PTGS) is a major mechanism regulating gene expression in higher eukaryotes. To identify novel players in PTGS, a forward genetics screen was performed on an Arabidopsis thaliana line ...Leer más >
Post-transcriptional gene silencing (PTGS) is a major mechanism regulating gene expression in higher eukaryotes. To identify novel players in PTGS, a forward genetics screen was performed on an Arabidopsis thaliana line overexpressing a strong growth-repressive gene, ETHYLENE RESPONSE FACTOR6 (ERF6). We identified six independent ethyl-methanesulfonate mutants rescuing the dwarfism of ERF6-overexpressing plants as a result of transgene silencing. Among the causative genes, ETHYLENE-INSENSITIVE5, SUPERKILLER2 and HASTY1 have previously been reported to inhibit PTGS. Notably, the three other causative genes have not, to date, been related to PTGS: UTP:RNA-URIDYLYLTRANSFERASE1 (URT1), C-TERMINAL DOMAIN PHOSPHATASE-LIKE3 (CPL3) and RESURRECTION1 (RST1). We show that these genes may participate in protecting the 3' end of transgene transcripts. We present a model in which URT1, CPL3 and RST1 are classified as PTGS suppressors, as compromisation of these genes provokes the accumulation of aberrant transcripts which, in turn, trigger the production of small interfering RNAs, initiating RNA silencing.< Leer menos
Palabras clave
CPL3
Palabras clave en inglés
RST1
small interfering RNA
Arabidopsis
Post-transcriptional gene silencing
URT1
Orígen
Importado de HalCentros de investigación