A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing.
NATRAN, Annelore
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
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Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
NATRAN, Annelore
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
VERCRUYSSE, Jasmien
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
GONZALEZ, Nathalie
Biologie du fruit et pathologie [BFP]
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Biologie du fruit et pathologie [BFP]
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
DUBOIS, Marieke
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
INZÉ, Dirk
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
< Reduce
Department of plant Biotechnology and Bioinformatics
Flanders Institute for Biotechnology
Language
en
Article de revue
This item was published in
Nature Plants. 2019, vol. 5, n° 5, p. 539-550
Nature Publishing Group
English Abstract
Post-transcriptional gene silencing (PTGS) is a major mechanism regulating gene expression in higher eukaryotes. To identify novel players in PTGS, a forward genetics screen was performed on an Arabidopsis thaliana line ...Read more >
Post-transcriptional gene silencing (PTGS) is a major mechanism regulating gene expression in higher eukaryotes. To identify novel players in PTGS, a forward genetics screen was performed on an Arabidopsis thaliana line overexpressing a strong growth-repressive gene, ETHYLENE RESPONSE FACTOR6 (ERF6). We identified six independent ethyl-methanesulfonate mutants rescuing the dwarfism of ERF6-overexpressing plants as a result of transgene silencing. Among the causative genes, ETHYLENE-INSENSITIVE5, SUPERKILLER2 and HASTY1 have previously been reported to inhibit PTGS. Notably, the three other causative genes have not, to date, been related to PTGS: UTP:RNA-URIDYLYLTRANSFERASE1 (URT1), C-TERMINAL DOMAIN PHOSPHATASE-LIKE3 (CPL3) and RESURRECTION1 (RST1). We show that these genes may participate in protecting the 3' end of transgene transcripts. We present a model in which URT1, CPL3 and RST1 are classified as PTGS suppressors, as compromisation of these genes provokes the accumulation of aberrant transcripts which, in turn, trigger the production of small interfering RNAs, initiating RNA silencing.Read less <
Keywords
CPL3
English Keywords
RST1
small interfering RNA
Arabidopsis
Post-transcriptional gene silencing
URT1
Origin
Hal importedCollections