Surface lipoproteome of Mycoplasma hominis PG21 and differential expression after contact with human dendritic cells
GORET, Julien
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
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USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
GORET, Julien
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
RENAUDIN, Hélène
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
BEBEAR, Cecile
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
PEREYRE, Sabine
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
< Leer menos
USC EA3671, Infections humaines à mycoplasmes et à chlamydiae
Laboratoire de Bactériologie
Idioma
en
Article de revue
Este ítem está publicado en
Future Microbiology. 2016, vol. 11, p. 179-194
Future Medicine
Resumen en inglés
<strong>Aim</strong>: To assess the lipoproteins that are involved in the interaction between Mycoplasma hominis and human dendritic cells. <strong>Materials & methods</strong>: The surface lipoproteome of M. hominis PG21 ...Leer más >
<strong>Aim</strong>: To assess the lipoproteins that are involved in the interaction between Mycoplasma hominis and human dendritic cells. <strong>Materials & methods</strong>: The surface lipoproteome of M. hominis PG21 was characterized by using Triton X-114 extraction and LC-MS/MS identification. The transcriptional changes in lipoprotein genes upon contact with human dendritic cells were determined by using reverse transcription quantitative PCR after identification of reference genes suitable for normalization. <strong>Results</strong>: A large-scale overexpression of lipoprotein genes was observed with 21 upregulated transcripts. Seven genes of unknown function were M. hominis species specific and six genes were putatively associated with increased nutrient capture from the host cell and adhesion. <strong>Conclusion</strong>: M. hominis regulates lipoprotein gene expression and may use species-specific mechanisms during the host colonization process.< Leer menos
Palabras clave
RT-qPCR
dendritic cells
expression
lipoprotein
Palabras clave en inglés
normalization
proteome
reference genes
mycoplasma hominis
Orígen
Importado de HalCentros de investigación