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Surface lipoproteome of Mycoplasma hominis PG21 and differential expression after contact with human dendritic cells

hal.structure.identifierUSC EA3671, Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierLaboratoire de Bactériologie
dc.contributor.authorGORET, Julien
hal.structure.identifierUSC EA3671, Infections humaines à mycoplasmes et à chlamydiae
dc.contributor.authorLE ROY, Chloé
hal.structure.identifierUSC EA3671, Infections humaines à mycoplasmes et à chlamydiae
dc.contributor.authorTOUATI, Arabella
hal.structure.identifierVirulence bactérienne et maladies infectieuses [VBMI]
dc.contributor.authorMESUREUR, Jennifer
hal.structure.identifierUSC EA3671, Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierLaboratoire de Bactériologie
dc.contributor.authorRENAUDIN, Hélène
hal.structure.identifierPlateforme de génomique [Institut Curie]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierUSC EA3671, Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierLaboratoire de Bactériologie
dc.contributor.authorBEBEAR, Cecile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierUSC EA3671, Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierLaboratoire de Bactériologie
dc.contributor.authorPEREYRE, Sabine
dc.date.issued2016
dc.identifier.issn1746-0913
dc.description.abstractEn<strong>Aim</strong>: To assess the lipoproteins that are involved in the interaction between Mycoplasma hominis and human dendritic cells. <strong>Materials & methods</strong>: The surface lipoproteome of M. hominis PG21 was characterized by using Triton X-114 extraction and LC-MS/MS identification. The transcriptional changes in lipoprotein genes upon contact with human dendritic cells were determined by using reverse transcription quantitative PCR after identification of reference genes suitable for normalization. <strong>Results</strong>: A large-scale overexpression of lipoprotein genes was observed with 21 upregulated transcripts. Seven genes of unknown function were M. hominis species specific and six genes were putatively associated with increased nutrient capture from the host cell and adhesion. <strong>Conclusion</strong>: M. hominis regulates lipoprotein gene expression and may use species-specific mechanisms during the host colonization process.
dc.language.isoen
dc.publisherFuture Medicine
dc.subjectRT-qPCR
dc.subjectdendritic cells
dc.subjectexpression
dc.subjectlipoprotein
dc.subject.ennormalization
dc.subject.enproteome
dc.subject.enreference genes
dc.subject.enmycoplasma hominis
dc.title.enSurface lipoproteome of Mycoplasma hominis PG21 and differential expression after contact with human dendritic cells
dc.typeArticle de revue
dc.identifier.doi10.2217/fmb.15.130
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalFuture Microbiology
bordeaux.page179-194
bordeaux.volume11
bordeaux.peerReviewedoui
hal.identifierhal-02630707
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02630707v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Future%20Microbiology&amp;rft.date=2016&amp;rft.volume=11&amp;rft.spage=179-194&amp;rft.epage=179-194&amp;rft.eissn=1746-0913&amp;rft.issn=1746-0913&amp;rft.au=GORET,%20Julien&amp;LE%20ROY,%20Chlo%C3%A9&amp;TOUATI,%20Arabella&amp;MESUREUR,%20Jennifer&amp;RENAUDIN,%20H%C3%A9l%C3%A8ne&amp;rft.genre=article


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