Electrochemical Atomic Force Microscopy Imaging of Redox-Immunomarked Proteins on Native Potyviruses: From Subparticle to Single-Protein Resolution.
BESONG-NDIKA, Jane
Biologie du fruit et pathologie [BFP]
Helsingin yliopisto = Helsingfors universitet = University of Helsinki
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Biologie du fruit et pathologie [BFP]
Helsingin yliopisto = Helsingfors universitet = University of Helsinki
Idioma
en
Article de revue
Este ítem está publicado en
ACS Nano. 2015, vol. 9, n° 5, p. 4911-4924
American Chemical Society
Resumen en inglés
We show herein that electrochemical atomic force microscopy (AFM-SECM), operated in molecule touching (Mt) mode and combined with redox immunomarking, enables the in situ mapping of the distribution of proteins on individual ...Leer más >
We show herein that electrochemical atomic force microscopy (AFM-SECM), operated in molecule touching (Mt) mode and combined with redox immunomarking, enables the in situ mapping of the distribution of proteins on individual virus particles and makes localization of individual viral proteins possible. Acquisition of a topography image allows isolated virus particles to be identified and structurally characterized, while simultaneous acquisition of a current image allows the sought after protein, marked by redox antibodies, to be selectively located. We concomitantly show that Mt/AFM-SECM, due to its single-particle resolution, can also uniquely reveal the way redox functionalization endowed to viral particles is distributed both statistically among the viruses and spatially over individual virus particles. This possibility makes Mt/AFM-SECM a unique tool for viral nanotechnology.< Leer menos
Palabras clave
electrochemical atomic force microscopy
Palabras clave en inglés
electrochemistry
functional nanosystems
potyvirus
virus imaging
virus nanocarriers
Orígen
Importado de HalCentros de investigación