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Electrochemical Atomic Force Microscopy Imaging of Redox-Immunomarked Proteins on Native Potyviruses: From Subparticle to Single-Protein Resolution.

hal.structure.identifierLaboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]
dc.contributor.authorNAULT, Laurent
hal.structure.identifierLaboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]
dc.contributor.authorTAOFIFENUA, Cécilia
hal.structure.identifierLaboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]
dc.contributor.authorANNE, Agnès
hal.structure.identifierLaboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]
dc.contributor.authorCHOVIN, Arnaud
hal.structure.identifierLaboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]
dc.contributor.authorDEMAILLE, Christophe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierHelsingin yliopisto = Helsingfors universitet = University of Helsinki
dc.contributor.authorBESONG-NDIKA, Jane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCARDINALE, Daniela
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCARETTE, Noelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMICHON, Thierry
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorWALTER, Jocelyne
dc.date.issued2015
dc.identifier.issn1936-0851
dc.description.abstractEnWe show herein that electrochemical atomic force microscopy (AFM-SECM), operated in molecule touching (Mt) mode and combined with redox immunomarking, enables the in situ mapping of the distribution of proteins on individual virus particles and makes localization of individual viral proteins possible. Acquisition of a topography image allows isolated virus particles to be identified and structurally characterized, while simultaneous acquisition of a current image allows the sought after protein, marked by redox antibodies, to be selectively located. We concomitantly show that Mt/AFM-SECM, due to its single-particle resolution, can also uniquely reveal the way redox functionalization endowed to viral particles is distributed both statistically among the viruses and spatially over individual virus particles. This possibility makes Mt/AFM-SECM a unique tool for viral nanotechnology.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subjectelectrochemical atomic force microscopy
dc.subject.enelectrochemistry
dc.subject.enfunctional nanosystems
dc.subject.enpotyvirus
dc.subject.envirus imaging
dc.subject.envirus nanocarriers
dc.title.enElectrochemical Atomic Force Microscopy Imaging of Redox-Immunomarked Proteins on Native Potyviruses: From Subparticle to Single-Protein Resolution.
dc.typeArticle de revue
dc.identifier.doi10.1021/acsnano.5b00952
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
dc.subject.halSciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique
bordeaux.journalACS Nano
bordeaux.page4911-4924
bordeaux.volume9
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-02641377
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02641377v1
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