NAULT, Laurent; TAOFIFENUA, Cécilia; ANNE, Agnès; CHOVIN, Arnaud; DEMAILLE, Christophe; BESONG-NDIKA, Jane; CARDINALE, Daniela; CARETTE, Noelle; MICHON, Thierry; WALTER, Jocelyne

doi:10.1021/acsnano.5b00952

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

NAULT, Laurent
Laboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]

TAOFIFENUA, Cécilia
Laboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]

ANNE, Agnès
Laboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]

Langue

Article de revue

Ce document a été publié dans

ACS Nano. 2015, vol. 9, n° 5, p. 4911-4924

American Chemical Society

Résumé en anglais

We show herein that electrochemical atomic force microscopy (AFM-SECM), operated in molecule touching (Mt) mode and combined with redox immunomarking, enables the in situ mapping of the distribution of proteins on individual virus particles and makes localization of individual viral proteins possible. Acquisition of a topography image allows isolated virus particles to be identified and structurally characterized, while simultaneous acquisition of a current image allows the sought after protein, marked by redox antibodies, to be selectively located. We concomitantly show that Mt/AFM-SECM, due to its single-particle resolution, can also uniquely reveal the way redox functionalization endowed to viral particles is distributed both statistically among the viruses and spatially over individual virus particles. This possibility makes Mt/AFM-SECM a unique tool for viral nanotechnology.< Réduire

Mots clés

electrochemical atomic force microscopy

Mots clés en anglais

electrochemistry

functional nanosystems

potyvirus

virus imaging

virus nanocarriers