A fluorometric assay for trehalose in the picomole range
GIBON, Yves
Biologie du fruit et pathologie [BFP]
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
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Biologie du fruit et pathologie [BFP]
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
GIBON, Yves
Biologie du fruit et pathologie [BFP]
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
< Reduce
Biologie du fruit et pathologie [BFP]
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
Language
en
Article de revue
This item was published in
Plant Methods. 2013, vol. 9, n° 1, p. 21
BioMed Central
English Abstract
The fluorometric assay is highly specific for trehalose and sensitive enough to measure the trehalose content of very small amounts of plant tissue. Chilling induced a 2-fold accumulation of trehalose in A. thaliana rosettes, ...Read more >
The fluorometric assay is highly specific for trehalose and sensitive enough to measure the trehalose content of very small amounts of plant tissue. Chilling induced a 2-fold accumulation of trehalose in A. thaliana rosettes, but the levels were too low to make a substantial quantitative contribution to osmoregulation. Trehalose is unlikely to function as a signal of sucrose status. The abnormal inflorescence branching phenotype of the maize ramosa3 mutant might be linked to a decrease in trehalose levels in the inflorescence primordia or a downward shift in the trehalose:Tre6P ratio.Read less <
Keywords
ramosa3
English Keywords
arabidopsis thaliana
trehalase
trahalose
zea mays
Origin
Hal importedCollections