ANNE, Agnès; CHOVIN, Arnaud; DEMAILLE, Christophe; MICHON, Thierry

doi:10.1007/978-1-4939-7808-3_30

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ANNE, Agnès
Université Paris Diderot - Paris 7 [UPD7]

CHOVIN, Arnaud
Université Paris Diderot - Paris 7 [UPD7]

DEMAILLE, Christophe
Université Paris Diderot - Paris 7 [UPD7]

Langue

Chapitre d'ouvrage

Ce document a été publié dans

Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols, Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols. 2018, vol. 1776

Humana Press

Résumé en anglais

We present in this chapter a new experimental approach allowing the high resolution imaging of immune complexes on virus particles. Combined atomic force-electrochemical microscopy (AFM-SECM) is used to image the presence of ferrocene functionalized specific antibodies on the surface of potyvirus particles. For this purpose, potyviruses, flexuous filamentous phytoviruses with a high aspect ratio, have been chosen. This technique allows analysis of the distribution of antibody labeling over the virus population. But, more importantly, it opens up the imaging of immune complexes decorating a single viral particle. Finally, its high resolution allows the characterization in situ of the ultrastructure of a single immune complex on the particle.< Réduire

Mots clés

Electrochemical atomic force microscopy

Lettuce mosaic virus (LMV)

Mots clés en anglais

Potyvirus

Redox antibodies

Single viral protein imaging

Viral nanoparticles

DOI

http://dx.doi.org/10.1007/978-1-4939-7808-3_30

Project ANR

Imagerie électrochimique fonctionnelle de systèmes enzymatiques multi-composants organisés sur des virus nano-gabarits - ANR-14-CE09-0009

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332