Redox-Immunofunctionalized Potyvirus Nanoparticles for High-Resolution Imaging by AFM-SECM Correlative Microscopy.
Language
en
Chapitre d'ouvrage
This item was published in
Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols, Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols. 2018, vol. 1776
Humana Press
English Abstract
We present in this chapter a new experimental approach allowing the high resolution imaging of immune complexes on virus particles. Combined atomic force-electrochemical microscopy (AFM-SECM) is used to image the presence ...Read more >
We present in this chapter a new experimental approach allowing the high resolution imaging of immune complexes on virus particles. Combined atomic force-electrochemical microscopy (AFM-SECM) is used to image the presence of ferrocene functionalized specific antibodies on the surface of potyvirus particles. For this purpose, potyviruses, flexuous filamentous phytoviruses with a high aspect ratio, have been chosen. This technique allows analysis of the distribution of antibody labeling over the virus population. But, more importantly, it opens up the imaging of immune complexes decorating a single viral particle. Finally, its high resolution allows the characterization in situ of the ultrastructure of a single immune complex on the particle.Read less <
Keywords
Electrochemical atomic force microscopy
Lettuce mosaic virus (LMV)
English Keywords
Potyvirus
Redox antibodies
Single viral protein imaging
Viral nanoparticles
ANR Project
Imagerie électrochimique fonctionnelle de systèmes enzymatiques multi-composants organisés sur des virus nano-gabarits - ANR-14-CE09-0009
Origin
Hal importedCollections